When the relationships between the number of methylated genes and the clinicopathological parameters of ICC were examined among these 8 genes, an increased number of methylated genes was associated with male sex, lower T stage, BD or SD type, the absence of intraglandular and/or extraglandular mucin production, the absence of perineural invasion, the absence of BilIN, the presence of chronic liver disease, the absence of nodal metastasis, and lower TNM stage (Table 2). This evidence concerns the gene MUC5AC and Behcet disease.