Notably, Plekha5 was also mutated in PT cells (153PT: 10/18, 55.5%) and most often mutated in metastatic tumor cells (153LMT: 17/20, 85%), as revealed by single-cell sequencing (Fig. 4b, c; Supplementary Fig. 4c), but it failed to be detected in PT bulk sequencing (Fig. 1c; Table 1). This evidence concerns the gene PLEKHA5 and metastatic neoplasm.