Calr was knocked out using CRISPR/Cas9 gene editing in Huh7.5 cells, a human hepatocellular carcinoma-derived cell line (39) that secretes endogenous AAT (hereafter called WT or CRT KO, respectively, for the control Huh7.5 (transduced with an empty lentiviral vector) and CRISPR/Cas9-edited cells (transduced with sgRNA that targeted CRT)). Here, SERPINA1 is linked to hepatocellular carcinoma.