TNF and ischemia: Further, we observed that the pathways related to apoptosis, fibrosis, and adipogenesis were significantly enriched by ischemia; pro-apoptotic genes such as FAS, CAS2/6/7, PARP6/8/11/12/14, TNF, and TNFRSF1/10/10B/25, fibrosis-related genes, collagen (COL1/3/ 4/5/6/7/12//15/16/18), and adipogenesis-related genes were downregulated, suggesting the existence of a protective process from cell damage against ischemia injury in the kidney tissue.