The comparison between reperfusion and pre-ischemia (reperfusion versus pre-ischemia) conditions showed that reperfusion induced changes in various pathways related to lipid and drug metabolism such as nicotine, serotonin, and melatonin degradation, FXR/RXR, and PXR/RXR signaling pathway, consistent with the results between ischemia and pre-ischemia conditions (Fig. 3c and Supplementary Table 8). This evidence concerns the gene NR1I2 and ischemia.