As shown in Figure 3, HAND2-AS1 overexpression did not significantly affect expression levels of PI3K and AKT, but significantly promoted the phosphorylation of PI3K and AKT in cells of two NSCLC cell lines NCI-H23 and NCI-H522, but not in cells of normal lung tissue cell lineBEAS-2B (data not shown). The gene discussed is HAND2; the disease is non-small cell lung carcinoma.