In the present study, HAND2-AS1 overexpression in cells of two NSCLC cell lines NCI-H23 and NCI-H522 and one normal lung tissue cell line BEAS-2B, and the effects of HAND2-AS1 overexpression on PI3K/Akt-related proteins were explored by Western blot. The gene discussed is AKT1; the disease is non-small cell lung carcinoma.