Using such a framework for simulating perturbation of TF expression as well as information flow in the cellular circuitry, we fitted our regulatory models to 32 brain tumor cell lines (using a training‐validation set split of 24‐8 and 4‐fold cross‐validation) in the CCLE cell line collection (Barretina et al, 2012), which can each be assigned an expression subtype based on proximity to centroids extracted from TCGA tumor data (Fig EV3A and B, see Materials and Methods for details). The gene discussed is TF; the disease is neoplasm.