The Twist1 and Wnt/β-catenin signaling pathways play important roles in the development of osteosarcoma, in which Twist1 promotes the expression of specific cell surface receptors in osteosarcoma cells via activating Tcf4-mediated Wnt/β-catenin signaling pathway (Wu et al., 2014). This evidence concerns the gene TCF4 and osteosarcoma.