Basing on the expression of CDKL3 and ATG5 in ESCC tissues, 103 patients were further divided into 4 groups including group A (CDKL3-high and ATG5-high), group B (CDKL3-high and ATG5-low), group C (CDKL3-low and ATG5-high) and group D (CDKL3-low and ATG5-low). This evidence concerns the gene CDKL3 and esophageal squamous cell carcinoma.