In 28 non-metastatic primary breast cancers, H-IRS was observed in nine cases (32%) and L-IRS in 19 (68%) while in 26 metastatic primary breast cancers, H-IRS was observed in 20 (77%) breast cancers and L-IRS in 6 (23%) (Figure 3). The gene discussed is IARS1; the disease is breast cancer.