These results were consistent with the quantitative analysis of RNA:m5C methyltransferases expression profile in gliomas, indicating that the expression levels of NOP2, NSUN2, NSUN4, NSUN5, and NSUN7 were shown to be positively correlated with the malignant progression of glioma, while the expression of NSUN6 were negatively correlated with glioma. The gene discussed is NSUN4; the disease is glioma.