In this study, we comprehensively studied the expression profiles of the NOL1/NOP2/sun domain RNA methyltransferase family, which are RNA:m5C methyltransferases, using the RNA sequencing data from the CGGA (n = 306) and TCGA (n = 616) datasets and aimed to investigate its prognostic value in glioma. Here, NOP2 is linked to central nervous system cancer.