In order to determine the role of cell communication on therapeutic efficacy, we utilized metastatic melanoma (1205Lu) cells that we previously generated to overexpress either 1) wild type connexin 43 (1205LuC), 2) a dominant negative connexin 43 that blocks both coupling with the mutant as well as wild type coupling through heterotypic gap junction channels (1205LuT) as well as the plasmid control (1205LuP) which has very low endogenous coupling [26]. This evidence concerns the gene GJA1 and metastatic melanoma.