CD4 and colorectal carcinoma: Flow cytometric analysis of single cell suspensions prepared from 5 CRC patients indicated that CD25+CD127low Tregs comprised approximately 25% of total CD45+CD2+CD4+ T cells (Figure 6B), which is similar to that reported previously for CRC (20) and much higher than in surrounding uninvolved tissue (Supplementary Figure S7) or healthy donor peripheral blood (Figure 6C).