Consistent with SA-β-gal staining, there was higher expression of HP1β, p21, and p16 proteins and mRNA, and lower expression of Lmnb1 mRNA in the kidneys of CKD mice on a high Pi diet compared to CKD mice on a normal Pi diet (Supplemental Figure 6B and Figures 5E, F). Here, TFF3 is linked to chronic kidney disease.