Pretreatment with TFAM also significantly reduced the activity of LDH and the MDA content, increased the serum levels of NO, and upregulated the cerebral mRNA expression of inducible nitric oxide synthase (iNOS) in rats with ischemia–reperfusion-induced cerebral infarction subjected to occlusion of the right middle cerebral artery in comparison with nimodipine (2 mg/kg) as a control (Wen and Chen, 2007). This evidence concerns the gene NOS2 and cerebral infarction.