To this end, a 1 × 105 ALDHhigh subpopulation of PC-3M cells expressing EGFP or cyclin A1-EGFP vectors that were sorted by FACS Aria were intra-cardiacally implanted into nude mice, and bone metastasis was allowed to establish for 30 days after tumor inoculation. The gene discussed is CCNA1; the disease is neoplasm.