We further determined whether disulfiram treatment alters the inflammatory responses in the heart at both day 14 and day 21 post infection, we evaluated B. burgdorferi induced myocardial inflammation by quantification of mRNA transcripts of CxCL1 (KC), CxCL2 (MIP-2), CCL5 (RANTES), TNF, IFNγ, IL-10, IL-1β, iNOS/NOS-2 by qRT-PCR. Here, TNF is linked to infection.