OTX2 and retinoblastoma: Only the lymph node metastasis and HPG-RBG1 cells showed a high-level amplification (>20×) in 14q harboring OTX2 (a gene that has been described as amplified in retinoblastoma [11]), as well as other focal gains or amplifications in 2q, 11q and 14q that did not include cancer-related genes.