According to the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis, the DEGs were mainly enriched in Alcoholism, Axon guidance, Regulation of actin cytoskeleton, Cell cycle, Hedgehog signaling pathway, MAPK signaling pathway Proteoglycans in cancer and mTOR signaling pathway (Table S1). The gene discussed is MTOR; the disease is alcohol dependence.