The most methylated genes found in colon primary tumour compared to LNM were OPRL1, NUDT11, STAC2, LLfos-48D6.2 and RP11-281J9.2. GO analysis showed that shared hypermethylated genes in primary tumour and LNM were mainly related to synaptic and neural activity. Here, OPRL1 is linked to neoplasm.