Three myelomeningocele subjects (two females and one male) carried the three RDVs NC_000023.10:g.48371088G>A p.(Gly223Ser), NC_000023.10:g.48371104G>A p.(Arg228His), and NC_000023.10:g.48374165C>G p.(Ala336Gly), all in highly conserved functional regions of PORCN [37]. Here, PORCN is linked to myelomeningocele.