ICI combinations significantly increased grade 1–2 rash (29.8% vs. 13.5%, P < 0.001), hypothyroidism (24.1% vs. 15.8%, P = 0.015), hyperthyroidism (16.1% vs. 3.9%, P < 0.001), myocarditis (10.6% vs. 0.9%, P < 0.001), hyperglycemia (28.6% vs. 3.7%, P < 0.001), amylase/lipase increase (23.5% vs. 4.0%, P < 0.001), Aspartate aminotransferase [AST] increase (36.5% vs. 11.2%, P < 0.001), Alanine aminotransferase [ALT] increase (35.1% vs. 12.2%, P < 0.001) and hypercreatine (12.5% vs. 2.9%, P < 0.001; Supplementary Material, Table S1). This evidence concerns the gene GPT and hyperthyroidism.