There was also a meningioma cell cluster enriched for CDH2 and PTPRZ1 that was present after 12 h in 3D monoculture, but not after 14 days (Supplementary Fig. 6d), suggesting that neither 2D nor 3D monoculture conditions accurately recapitulate the in vivo molecular heterogeneity of meningioma cells. This evidence concerns the gene PTPRZ1 and meningioma.