CD28 and hepatocellular carcinoma: Dual-targeting CAR-T cells coexpressing GPC3 and asialoglycoprotein receptor 1 (ASGR1, a liver tissue-specific protein) targeting CAR’s featuring CD3 zeta chain (CD3ζ) and 28BB (containing both CD28 and 4-1BB signaling domains) have been tested in a GPC3+ASGR1+ HCC tumor xenograft mouse model31.