TNF and melanoma: Therefore, to figure out whether A20 was involved in the regulation of cell death, we adopted positive controls of melanoma cell apoptosis, necroptosis, and pyroptosis induced by TS (TNF-α and SM-164), TSZ (TNF-α, SM-164 and Z-VAD-FMK), and FC (FeSO4 and CCCP) respectively as described previously21–23.