PTGS2 and melanoma: By luciferase reporter assays, we detected a significant decrease in luciferase activity upon melanoma cells transfection with the pLightSwitch-3’UTR-PTGS2 vector together with the miR-146a mimic when compared with controls, confirming a role for miR-146a in the post-transcriptional regulation of PTGS2 (supplementary Fig. S2A).