DNMT3A and acute myeloid leukemia: Next, we performed multivariate analysis that included age, established clinical risk (subgroup), gender and missense mutations of RUNX1, DNMT3A and PTPN11. The results show that the gender variable has a minor impact on the overall survival, established clinical risk (subgroup), age, while the missense mutations of DNMT3A play a major role in the progression and aggressiveness of AML (Fig. 5d).