Here, the recombinant plasmid of co-expression of CAMPs and IL-4/6 was firstly constructed and entrapped with chitosan nanoparticles, which is used as a potential replacement of antibiot Many indicators consolidated that both of innate and adaptive immunity were enhanced, and the immunity against bacterial infection was also elevated for one month or so, which probably could not protect the mice in a medium- or long-term due to the limited existence of recombinant plasmid in vivo. This evidence concerns the gene IL4 and bacterial infectious disease.