In their first report in 2007, the authors utilized the human cytokine array method (combining the individual assets of ELISA, enhanced chemiluminescence, and the high-throughput of microspot) in order to detect simultaneously multiple cytokines in the plasma of 20 therapy-naïve MPN patients (N = 10 PMF, N = 5 PV, and N = 5 ET) and N = 4 controls, detecting selectively higher levels of Tissue Inhibitor of Metalloproteinase (TIMP-1), MIP-1, and Insulin-like Growth Factor Binding Factor-2 (IGFBP-2) in PMF patients as compared to ET, PV, and controls [67]. Here, TIMP1 is linked to acquired polycythemia vera.