We first evaluated in three AML cell lines, HL-60, THP1 and U937, the basal expression and activation of the Wnt molecules, including total β-catenin, pan-phosphorylated β-catenin(Ser33–37/Thr41), Ser675-phospho-β-catenin, active non-phospho-β-catenin, GSK-3β (total and Ser9) and GSK-3α (total and Ser9). This evidence concerns the gene GSK3B and acute myeloid leukemia.