To assess the direct functional role of Arid1a without other compensatory molecular events appearing during autochthonous progression in the “KAC” GEM model, we employed CRISPR-Cas9 based approach to delete this gene in the Arid1a-expressing PDAC lines established from spontaneous PDAC arising in “KC” and “KPC” (LSL-KrasG12D/+; LSL-Trp53R172H/+; Pdx-1-Cre) mice. Here, ARID1A is linked to keratoconus.