Based on the ROC curve, the cut-off value for plasma APE1/Ref-1 level for diagnosis of atherosclerosis in ApoE−/− mice fed with a Western-type diet (WD) as compared with wild-type control mice (WT) was set at 4.90 ng/mL, with an area under the ROC curve of 1.0, a sensitivity of 100%, and a specificity of 91% (Figure 5B). The gene discussed is APOE; the disease is atherosclerosis.