Finally, we evaluated the sensitivity and effectiveness of the custom NGS panel in detecting the main fusion genes associated with B-ALL patients: the ETV6/RUNX1 fusion gene (reported in 25% of children and 1–3% of adults), the BCR/ABL1 fusion gene (reported in 10% of children and 20% of adults), and MLL rearrangements (reported in 6% of children and 9% of adults) [1,2,35]. This evidence concerns the gene ABL1 and acute lymphoblastic leukemia.