To investigate whether nestin+ BM cells are affected in human AML, we performed immunohistochemistry for the human NESTIN protein in BM biopsies from MLL-AF9+ (n = 5) or MLL-AF9− (n = 56) AML patients and control donors (n = 12) (Figures 1A–1C). Here, NES is linked to acute myeloid leukemia.