To identify EMT regulators that are involved in the p53-dependent effect of KLF5 on liver cancer cell EMT, we first tested the expression levels of the known EMT activators Snail, Slug, Twist, ZEB1 and ZEB2 in HepG2 and Hep3B cells after KLF5 overexpression and knock-down. This evidence concerns the gene TWIST1 and liver cancer.