Figure 2(a) shows representative dot blots for Annexin V/7-AAD staining. 7-AAD single-stained cells were not detected, and most stained cells were Annexin V single or Annexin V and 7-AAD double stained. These data suggest that CME induced apoptotic cell death in human and mouse breast cancer cells. In addition, Annexin V-positive cells increased with increasing doses of CM-EE. To confirm that CM-EE induces apoptotic cell death, the level of PARP and caspase 3/7 protein was measured using western blotting (Figure 2(b)). This evidence concerns the gene ANXA5 and breast carcinoma.