To further investigate the molecular mechanisms of the most effective dose of EECGL (EECGLH, revealed by the above statistically significant data level) on HM (the most significant observations were observed from the above data and statistical significant level) induced changes, in attenuating the progression of NAFLD, protein and mRNA expression levels of several apoptosis-related proteins and inflammation-related genes expressions were determined by Western blot analysis and RT-PCR (Fig. 7). The gene discussed is FAM215A; the disease is metabolic dysfunction-associated steatotic liver disease.