To estimate whether high-dose-IR and Bip inhibition-treated cells have an effect of preventing the generation of glioma from GSCs as a vaccine in vivo, the syngenic C57BL/6J mice were administrated with lentivirus transfection- and/or IR-treated GL261s GSCs subcutaneously before untreated GL261s GSCs were implanted intracranially for tumor model establishment. Here, HSPA5 is linked to glioma.