A higher number of genes were identified when depleted gRNAs were overlapped between the two cell lines (Fig. 6C,D) several of which (i.e., BOP1, RSF1, ACTL6A, ASH2L, and ATR) showed high copy number amplification or increase in expression in breast and lung cancer, suggesting that those genes might play essential roles in cancer proliferation and viability. This evidence concerns the gene ACTL6A and lung cancer.