Sinomenine significantly inhibited caspase-3 activity and the viability of B16-F10 mouse melanoma cells and induced apoptosis and related proteins expression (Bax/Bcl-2 ratio) at 25, 50 and 100 μM in a CCK-8 assay and Annexin V stained cell counting as well as in Western blot analysis [45]. This evidence concerns the gene BAX and melanoma.