All these studies performed using brain organoids/neurospheres suggest that 3D culture may be a better system to reproduce AD in vitro, as it allows for further maturation of the cells and hampers the outflow of secreted proteins, enhancing the aggregation of Aβ and Tau, better reflecting the in vivo situation, and allowing to study the relation between Aβ and Tau pathologies. This evidence concerns the gene MAPT and Alzheimer disease.