To further investigate the possible role of paracrine components in the process of synergistic crosstalk in the interaction of cancer and stroma cells in pro-inflammatory multicellular-TME, we next analyzed TGF-β3 and TNF-β expression in HCT116 to find out whether TGF-β is implicated in the boost of tumor cell proliferation and tumor promoting factors. This evidence concerns the gene TGFB1 and neoplasm.