First, we investigated the efficacy of resveratrol and/or Sirt1-ASO 3D-alginate HCT116 cultures in TNF-β or in multicellular-TME, mimicking the in vivo TME, and to elucidated the involvement of paracrine therapeutic mediators in intercellular crosstalk and on tumor cell proliferation and cell viability. This evidence concerns the gene LTA and neoplasm.