TGFB3 and neoplasm: We also found intensive cross-talk in multicellular-TME during tumor co-cultures and increased TGF-β3 and TNF-β expression in HCT116 cells, underlining active TGF- β3 and TNF-β signaling as paracrine interactions between T-lymphocytes, fibroblasts and HCT116 cells in the TME.