(3) Interestingly, we found that resveratrol suppressed the proliferation, colony formation and invasion of CRC cells in 3D alginate cultures in multicellular-TME, and this was effectively blocked by the specific knockdown of Sirt1 through ASO-Sirt1, pointing to the fact that Sirt1 suppression at the mRNA level is not reversible, emphasizing the crucial role of this enzyme. The gene discussed is SIRT1; the disease is colorectal carcinoma.