Considering that activation of the pro-inflammatory transcription factor NF-κB signaling is one of the major pathway activations of pro-inflammatory TME in CRC cells [15,49], the HCT116 cells were cultured in 3D-alginate beads and co-cultured in the multicellular-TME, TNF-β-TME and then either left untreated or treated with various doses of resveratrol (1, 2, 5, 10 μM), or were transfected with Sirt1-SO, Sirt1-ASO (0.5 μM) in the presence of Lipofectin (10 μL/mL) and co-treated with resveratrol (5 μM) for 14 days in serum-starved medium, as described in the section Materials and Methods. Here, SIRT1 is linked to colorectal carcinoma.