It has been reported that pro-inflammatory cytokines enhance the growth and infiltration of tumor cells by the transcription factor NF-κB [48]; for this reason, we examined the expression and nuclear translocation of NF-κB related to the malignancy and survival of CRC cells and conducted an immunofluorescence labelling for p65-NF-κB, as described in the Materials and Methods section. This evidence concerns the gene NFKB1 and colorectal carcinoma.