SIRT1 and neoplasm: First, we investigated the efficacy of resveratrol and/or Sirt1-ASO 3D-alginate HCT116 cultures in TNF-β or in multicellular-TME, mimicking the in vivo TME, and to elucidated the involvement of paracrine therapeutic mediators in intercellular crosstalk and on tumor cell proliferation and cell viability.