Meanwhile, 4-branched H3K(+H)4b peptide with an additional histidine for a better endosomal escape not only exhibited comparable gene silencing effect as compared with H3K8b for in vitro Raf-1 siRNA transfection, but also good antitumoral activity comparable with H3K4b for intravenous administration of Raf-1 siRNA nanoplexes to MDA-MB-435 tumor-bearing mice [53]. The gene discussed is RAF1; the disease is neoplasm.