Firstly, we performed this assay in presence of HLA-G and PD-L1 and we found that both HLA-G and PD-L1 can block the interaction of immune cells with cancer cells, however, in-vitro HLA-G provided stronger immune protection to cancer cells compared to PD-L1 that initially resulted in increased lysis of the cancer cells. The gene discussed is CD274; the disease is cancer.