FOXM1 and neoplasm: To further assess the extent to which in vitro findings were representative of HCCs developed after DAA treatment, we analyzed by IHC the expression of Forkhead box protein M1 (FOXM1), Ki67 and vimentin (VIM) in thirteen surgically resected HCCs diagnosed within 12 months after completion of treatment with DAAs (post-DAA cohort) and in an active HCV cohort of 39 patients composed of 28 patients tested by RNA-seq and 11 randomly selected additional patients with clinical characteristics and tumor stage matched with the post-DAA cohort (as reported in Table 1).