CD274 and bone cancer: Local injection (deep tissue around tumor bone) of PD-L1 (2 or 5 μg) (15) in the PTD 21 mice significantly suppressed bone cancer–induced thermal hyperalgesia and mechanical allodynia (Figure 2, A and B, 2-way RM ANOVA, treatments: F[2, 20] = 21.59, P < 0.001, treatment × time: F[10, 100] = 3.30, P < 0.001 for PWL; treatments: F[2, 20] = 21.39, P < 0.001, treatment × time: F[10, 100] = 1.95, P = 0.04 for PWT).