Consistently, the analgesic effect of PD-L1 on bone cancer pain was weakened (allodynia) or eliminated (hyperalgesia) in SHP-1–CKO mice (Figure 6, E and F, 2-way RM ANOVA, treatments: F[1, 12] = 97.77, P < 0.001 for PWL; F[1, 12] = 20.52, P < 0.001 for PWT). The gene discussed is NR0B2; the disease is bone cancer.