We cultured tumor cell lines derived from H7;Lats1/2mut3;YAP/TAZmut4 mice — 459T cells — and transduced them with Ad-Cre to ablate residual YAP and TAZ. We optimized the virus concentration to minimize cytotoxicity and ensure at least 95% gene delivery for each transduction by monitoring GFP expression. Here, LATS1 is linked to neoplasm.