More importantly, RAB31WT, RAB31Q65L and RAB31R77Q (the mutant that functions similarly to RAB31Q65L in NSCLC), but not RAB313YF, could rescue the phenotype to promote endogenous EGFRM2, FLOT1, FLOT2, CD9, CD81 and CD63 in the concentrated conditional media (Fig. 6g), whereas all of these RAB31 forms including RAB313YF could rescue the decrease of these membrane proteins in cells with stable knockdown of endogenous RAB31 (Supplementary information, Fig. S9f). This evidence concerns the gene CD81 and non-small cell lung carcinoma.