As shown in Fig. 4a, silencing of REC8 expression in BGC823 and AGS-1 of gastric cancer cell promoted the phosphorylation of NF-κB p65 (Ser536), and no significance change of total NF-κB p65 was observed between shCTL and shREC8 (Additional file 1: Figure S1). This evidence concerns the gene NFKB1 and gastric cancer.