Among the 1751 DAC-treated single cells, random fractions of cells showed upregulation (UMI counts ≥ 3) of specific established tumor-suppressor genes methylation-silenced in colorectal cancers [23–25] (CHFR, FBLN2, ICAM4, and SFRP1), endogenous retroviruses (ERVs) (ERVMER61-1 and ERVW-1), and interferon-stimulated genes (IFI16 and IFI44) (Fig. 1d, e, f). Here, SFRP1 is linked to colorectal cancer.