The effects of α5-nAChR on cell proliferation, migration, and EMT can be witnessed in both EGFR wild-type (A549) and EGFR mutant (HCC827 and H1975) cell lines (Figure 4 and Figure 5); the animal experiment using HCC827 also proved that α5-nAChR can affect the tumor growth in EGFR-mutant cells (Figure 5C,D). Here, EGFR is linked to neoplasm.